Journal: Toxicology

Article Title: A 3D spheroid model of quadruple cell co-culture with improved liver functions for hepatotoxicity prediction.

doi: 10.1016/j.tox.2024.153829

Figure Lengend Snippet: Fig. 6 Drug-induced lipid dysregulation replicated by the 3D quadruple cell co- culture liver spheroid model. (A) Neutral fat visualization and quantification in the spheroid model after treatment of isoniazid, palmitic acid, nifedipine, or amiodarone via BODIPY™ 493/503 staining (n=3 for palmitic acid treatment, n=4 for other compounds). (B) Phospholipid imaging and quantification in the isoniazid or amiodarone-treated spheroid model through NBD-PE labeling (n=4). Scale bar: 200 μm. *p < 0.05, **p < 0.01, compared to the control group.

Article Snippet: In the test of drug-induced liver fibrosis risk, methotrexate (TMstandard, China) was adopted as the tested compound, and 48-hour TGF-β1 (MedChemExpress) treatment at a concentration of 10 ng/mL during Day 4-6 was selected for setting up the positive control.

Techniques: Co-Culture Assay, Staining, Imaging, Labeling, Control

Journal: PeerJ

Article Title: Small-scale screening of anticancer drugs acting specifically on neural stem/progenitor cells derived from human-induced pluripotent stem cells using a time-course cytotoxicity test

doi: 10.7717/peerj.4187

Figure Lengend Snippet: IC 50 values (µM) of drugs against hiPSC-NSPCs and hN-NSPCs.

Article Snippet: Cisplatin (Sigma-Aldrich), etoposide (Sigma-Aldrich), mercaptopurine (Sigma-Aldrich), and methotrexate (LKT Laboratories, St. Paul, MN, USA) were dissolved in dimethyl sulfoxide (DMSO) to generate 100 mM stock solutions.

Techniques:

Journal: Molecules

Article Title: Preparation of Polyvinyl Imine Modified Carbon Quantum Dots and Their Application in Methotrexate Detection

doi: 10.3390/molecules27165254

Figure Lengend Snippet: Interference of different substances on the determination of methotrexate.

Article Snippet: Methotrexate hydrate (>97%) was obtained from Shanghai Titan Technology Co., Ltd. (Shanghai, China).

Techniques: Concentration Assay